Yes, beginning with v2.7, ESET NOD32 is compatible with both the 32-bit and 64-bit versions of Microsoft Windows Vista. ESET NOD32 does not make changes or modifications to the kernel (the core of the operating system) in order to work under Windows Vista, so it is compatible with PatchGuard, Microsoft's technology to prevent the kernel from being modified under 64-bit versions of Windows.
After it has been installed, ESET NOD32 appears in the Malware Protection section of the Windows Security Center in Windows Vista, just as it does under earlier versions of Windows.